On Gluten and Celiac
Oct 20, 2025

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Introduction



What Foods Contain Gluten?


Celiac Disease



Gluten Challenge
 If you're going to be tested for Celiac disease, whether it's a blood test or an endoscopy, it's typically recommended you undergo what's known as a "gluten challenge". This involves eating a large amount of gluten for a period of time. Otherwise, you may end up with a false negative, and little to no consumption of gluten causes the small intestine to heal itself if you have Celiac. Sounds great right? Yes, but that leads to an inaccurate test for Celiac. Thus, a gluten challenge is initiated.

 Typically, a gluten challenge involves eating the equivalent of 4-5 slices of bread worth of gluten for a period of 2-4 weeks before testing for Celiac disease. But what does this actually mean? How much gluten can you consume besides bread?

 Let's assume a standard loaf of bread. Each slice weighs 29 g, and contains 3.1 g of protein. Since approximately 80% of protein in bread is made up of gluten, we can therefore conclude that 5 slices of bread contains approximately 12.4 g of gluten.

 In the table below, I will break down the amount of gluten contained in certain foods; per serving size, per 100 g, and per 100 calories. Note that data for the amount of gluten in specific grains is not always present. Therefore, I will be assuming the following:



Foods High in Gluten

Grains (Raw)
Food Serving Size Gluten
Per Serving
Gluten
Per 100 g
Gluten
Per 100 cal
Barley 1/4 cup (50 g) 2.5 g 5.0 g 1.4 g
Couscous 1/4 cup (56 g) 5.4 g 9.6 g 2.6 g
Farro 1/4 cup (45 g) 4.3 g 9.5 g 2.8 g
Rye 1/4 cup (42 g) 2.2 g 5.2 g 1.5 g
Spelt 1/4 cup (44 g) 4.8 g 11.0 g 3.3 g
Vital Wheat Gluten 1/4 cup (30 g) 22.6 g 75.2 g 20.3 g
White Wheat 1/4 cup (31 g) 2.4 g 7.7 g 2.1 g
Whole Wheat 1/4 cup (31 g) 3.1 g 9.9 g 2.9 g


Other
Food Serving Size Gluten
Per Serving
Gluten
Per 100 g
Gluten
Per 100 cal
Beer 12 oz (360 g) 1.1 g 0.3 g 0.7 g
Marmite 1/2 tsp (4 g) 1.0 g 25.0 g 10.0 g
Soy Sauce
(Shoyu)
1 tbsp (15 g) 0.9 g 6.1 g 11.5 g


Highest Sources of Gluten
Per serving: [TODO] - [TODO] mg ([TODO])
Per 100 g: [TODO] - [TODO] mg
Per 100 cal: [TODO] - [TODO] mg



Sources
  1. Nutrition Value
  2. FlatIcon
  3. Wikipedia - Gluten
  4. Celiac Disease Foundation - Sources of Gluten
  5. Reddit - Gluten Levels in Different Foods
  6. PubMed - Analyzing Gluten Content in Various Food Products Using Different Types of ELISA Test Kits
  7. Science Direct - Development of a barley reference material for gluten analysis
  8. PubMed - Characterization of rye flours and their potential as reference material for gluten analysis
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